منابع مشابه
Protein analysis by hydrogen exchange mass spectrometry.
Mass spectrometry has provided a powerful method for monitoring hydrogen exchange of protein backbone amides with deuterium from solvent. In comparison to popular NMR approaches, mass spectrometry has the advantages of higher sensitivity, wider coverage of sequence, and the ability to analyze larger proteins. Proteolytic fragmentation of proteins following the exchange reaction provides moderat...
متن کاملMinimizing back exchange in the hydrogen exchange-mass spectrometry experiment.
The addition of mass spectrometry (MS) analysis to the hydrogen exchange (HX) proteolytic fragmentation experiment extends powerful HX methodology to the study of large biologically important proteins. A persistent problem is the degradation of HX information due to back exchange of deuterium label during the fragmentation-separation process needed to prepare samples for MS measurement. This pa...
متن کاملHydrogen exchange-mass spectrometry: optimization of digestion conditions.
The direct linkage between folded structures of proteins and their function has increased the need for high resolution structures. In addition, there is a need for analytical methods for detecting and locating changes in the folded structures of proteins under a wide variety of conditions. The rates at which hydrogens located at peptide amide linkages undergo isotopic exchange has become the ba...
متن کاملHydrogen exchange mass spectrometry for the analysis of protein dynamics.
Hydrogen exchange coupled to mass spectrometry (MS) has become a valuable analytical tool for the study of protein dynamics. By combining information about protein dynamics with more classical functional data, a more thorough understanding of protein function can be obtained. In many cases, protein dynamics are directly related to specific protein functions such as conformational changes during...
متن کاملAnalyzing protein dynamics using hydrogen exchange mass spectrometry.
All cellular processes depend on the functionality of proteins. Although the functionality of a given protein is the direct consequence of its unique amino acid sequence, it is only realized by the folding of the polypeptide chain into a single defined three-dimensional arrangement or more commonly into an ensemble of interconverting conformations. Investigating the connection between protein c...
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ژورنال
عنوان ژورنال: Molecular & Cellular Proteomics
سال: 2002
ISSN: 1535-9476
DOI: 10.1074/mcp.m100009-mcp200